GLUT3 SPEC PDF

Compare Anti-Glucose Transporter GLUT3 antibody ab from Abcam on Be the first to write a review! Rabbit polyclonal to Glucose Transporter GLUT3. C5 and T4 cells showed the highest rate of GLUT-3 expression. These cells were ORL J Otorhinolaryngol Relat Spec. ;– glucose transporter 3 (GLUT3)2 is the major GLUT subtype in .. platelets without additional Ca2 at 20 °C in a F fluorescence spec-.

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Author manuscript; available in PMC Aug 1. The end point used in the real time RT-PCR quantification were the C T values, defined as the PCR cycle number in which the fluorescence generated by the amplification crosses the threshold. Growth factor signalling networks in the breast cancer and resistance to endocrine agents: Russo J, Russo IH.

After the solidification of collagen, the cells were fed with DMEM medium with daily intervals for 8 days. In this model, different stages of tumor initiation and progression are represented, MCFF being the normal stage, E2 cells the transformed stage by estrogen, C5 cells, the invasive stage, and T4 cells the tumorigenic stage.

J Natl Cancer Inst. CA Cancer J Clin. Am J Physiol Endocrinol Metab. Glut1 overexpression has been widely observed in many cancers including breast, lung, renal cell RCCcolorectal and pancreas carcinoma specimens; some, mostly Glut1, 3 are being found to be related to metastasis and poor prognosis [ 410 ]. It is well known that the members of EGFR family play important role in regulating a number of cellular processes, differentiation and malignant transformation [ 61 ].

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Lieberman M, Marks AD. Colony formation of represented cells in Agar methocel right column. Differential regulation of glucose transporter expression by estrogen and progesterone in Ishikawa endometrial cancer cells.

Jpn J Cancer Res. EGFR signaling pathway in breast cancers: Regulation of Warburg effect in early-passage breast cancer cells. Cellular transformation under several oncogenic stimuli and carcinogenic progression lead to altered substrate metabolism, increased aerobic glycolysis sprc excessive glucose consumption [ 56 ].

From the nine tumors obtained from C5 cells, four tumoral cell lines T4 were derived. Therefore, differentiation of mammary epithelium may be reversely associated with Glut1 and Glut12 expression. Although increased glucose metabolism and overexpression of Gluts in cancer are well known, glyt3 for tumor and stage-specific alterations as seen at Fig.

On the other hand, both carcinogenic initiators may give rise to different types of GLUT expressions. On respiratory impairment in cancer cells. Support Center Support Center. Epithelial to mesenchymal transition in human breast epithelial cells transformed by 17beta-estradiol. Two-tailed unpaired t test was used for gene expression analysis.

Hypoxia and adaptive landscapes in the evaluation of carcinogenesis. Mammalian cells use a wide variety of plasma-membrane sped systems in order to translocate several substrates into the cell.

Similarly, in glt3 study, long-term treatment with E2 showed increased Glut12 levels in MCF-7 cells [ 4 ]. New insights into estrogen receptor function in human breast cancer. T4-cancer cells were then isolated from these tumors [ 38 ].

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A family of glucose transporters GLUTs facilitates glucose movement across the plasma membranes in a tumor-specific manner. Methods GLUT expression patterns were investigated in an in vitro—in vivo progressive, estrogen-induced, mammary carcinogenesis model which consisted of four cell lines, with same genetic background.

Statistical analysis Two-tailed unpaired t test was used for gene expression analysis. The expression of Glut3 in the E2 cell was 1. It has been also observed that more advanced tumor stages are associated with more increased glucose uptake.

GLUT – The OpenGL Utility Toolkit

A family of glucose transporters GLUTs facilitates the movement of glucose across the plasma membranes in a tissue-specific manner, dependent on concentration gradient. On an in vitro basis, GLUT expression patterns have been most extensively studied in breast cancer cell lines [ 4 ].

The quality and quantity of each sample were verified by spectrophotometry using NanoDrop 3. A prospective study of endogenous estrogens and breast cancer in postmenopausal women.